CellKrafting
Explore cutting-edge insights in cell culture, biotechnology, and diagnostics with Cellkraft Biotech’s cell culture blog.
Topics
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Subculture Protocol for Adherent Cells
A highly simplified subculture protocol for most adherent cell lines
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What’s New in Animal Cell Culture?
1. Enzyme-Free, Automated Cell Detachment — A Biomanufacturing Breakthrough Traditional enzymatic detachment methods such as trypsinization can damage proteins on the cell surface and affect viability.A new 2025 development introduces a non-enzymatic, electrically-controlled detachment system, allowing the release of anchored cells using low-frequency alternating current on specially engineered surfaces. Why this matters 2. Deeper Control…
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Useful cell culture info
Details on Different Sizes of Cell Culture Dishes and Flasks Cell culture dishes and flasks come in various sizes. Below are some useful numbers such as surface area, seeding density, and volumes of media & reagent solutions for different culture vessels: Culture plate Surface Area (cm²) Seeding Density Cells at Confluency Trypsin 0.05% trypsin (mL)…